Team:Caltech/Week 11
From 2010.igem.org
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==Monday 8/23== | ==Monday 8/23== | ||
+ | *Ligate L90, L91, and L92 (overnight ligation) | ||
==Tuesday 8/24== | ==Tuesday 8/24== | ||
+ | *Transform the ligation product from yesterday | ||
==Wednesday 8/25== | ==Wednesday 8/25== | ||
+ | *Pick colonies from the transformed cells. Perform colony PCR and run gel on the PCR product. | ||
+ | **Gel shows negative result Failed | ||
+ | *Set up HSP-LacI-GFP experiment with glucose concentration (.1mM, .5mM, 1mM, 5mM) | ||
==Thursday 8/26== | ==Thursday 8/26== | ||
+ | *Glucose appears to not effect the GFP expression in this experiment which contradicts the result from previous experiment. | ||
==Friday 8/27== | ==Friday 8/27== |
Revision as of 20:09, 31 August 2010
People
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Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15
Monday 8/23
Tuesday 8/24
Wednesday 8/25
Thursday 8/26
Friday 8/27
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