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Team:Newcastle/18 August 2010
From 2010.igem.org
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| - | =Miniprep of | + | =Miniprep of filamentous cell part= |
==Aims== | ==Aims== | ||
| - | The aim of the experiment is to | + | The aim of the experiment is to prepare stocks of the plasmid DNA containing our filamentous cell part. |
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==Materials and Protocol== | ==Materials and Protocol== | ||
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==Discussion== | ==Discussion== | ||
| - | The concentration of the different | + | The concentration of the different tubes range from 140.0 µl/ml to 456.6 µl/ml. The standard value for miniprep extraction is 150 µg/ml. |
==Conclusion== | ==Conclusion== | ||
| - | High concentration | + | High DNA concentration was obtained. |
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 23:53, 27 October 2010
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Contents |
Miniprep of filamentous cell part
Aims
The aim of the experiment is to prepare stocks of the plasmid DNA containing our filamentous cell part.
Materials and Protocol
Please refer to protocols mentioned below for materials required:
Results
| yneA 1 | yneA 2 | yneA 3 | yneA 4 | |
|---|---|---|---|---|
| Concentration of DNA ng/µl | 247.3 | 233.6 | 456.6 | 140.0 |
Table 1: Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.
Figure 1: Screenshot of yneA tube 1
Figure 2: Screenshot of yneA tube 2
Figure 3: Screenshot of yneA tube 3
Figure 4: Screenshot of yneA tube 4
Go back to our main Lab book page
Discussion
The concentration of the different tubes range from 140.0 µl/ml to 456.6 µl/ml. The standard value for miniprep extraction is 150 µg/ml.
Conclusion
High DNA concentration was obtained.
   
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