"
BIOTEC Dresden/Notepad/1 October 2010
From 2010.igem.org
(Difference between revisions)
| (6 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{Biotec_Dresden/Header}} | ||
| + | <html> | ||
| + | <head> | ||
| + | <style type="text/css"> | ||
| + | #bodyContent p, #bodyContent pre, #bodyContent table { | ||
| + | margin:10px 30px; | ||
| + | } | ||
| + | </style> | ||
| + | </head> | ||
| + | </html> | ||
| + | |||
'''Assembly of the amplifier-reporter part''' | '''Assembly of the amplifier-reporter part''' | ||
| Line 4: | Line 15: | ||
The final aim is to ligate the product with part one in the order 1-45-26 to produce the amplifier-reporter: | The final aim is to ligate the product with part one in the order 1-45-26 to produce the amplifier-reporter: | ||
| - | ptetR RBS LuxR Stop1 Stop2 luxRp | + | ptetR RBS LuxR Stop1 Stop2 luxRp-------RBS RFP---------RBS-luxI-Stop1-Stop2 |
Ligation product showed the expected length in a restriction analysis also sequencing results expected. | Ligation product showed the expected length in a restriction analysis also sequencing results expected. | ||
| + | |||
| + | '''AHL Sensor''' | ||
| + | |||
| + | Amplification of the backbone by using different primers. | ||
| + | [[Category:BIOTEC Dresden/Notepad/October|October]] | ||
| + | {{Biotec_Dresden/month}} | ||
| + | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 22:56, 27 October 2010
Assembly of the amplifier-reporter part
Part 45 (gel purified after restriction) was ligated into the linearized vector containing part 46 using the standard assembly method. The final aim is to ligate the product with part one in the order 1-45-26 to produce the amplifier-reporter:
ptetR RBS LuxR Stop1 Stop2 luxRp-------RBS RFP---------RBS-luxI-Stop1-Stop2
Ligation product showed the expected length in a restriction analysis also sequencing results expected.
AHL Sensor
Amplification of the backbone by using different primers.
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
