BIOTEC Dresden/Notepad/21 September 2010

(Difference between revisions)

Latest revision as of 22:48, 27 October 2010

Please enable Javascript in your browser to get or upgrade to a browser that supports Javascript to get full experience of our Team Wiki

Parts Assembly

PCR amplification of the following parts and the plasmid backbone containing chloramphenicol was done.

4a, 9b, 14f, 20f, 21f

The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated.

Fusion Protein

Ligation of the parts was carried out followed by their transformation. Gradient PCR for the fusion parts was done.

@@ Line 17: / Line 17: @@
 The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated.
 '''Fusion Protein'''
-A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.
+Ligation of the parts was carried out followed by their transformation.
+Gradient PCR for the fusion parts was done.
-<hr>
+[[Category:BIOTEC Dresden/Notepad/September|September]]
 {{Biotec_Dresden/month}}
 {{Biotec_Dresden/Bottom}}