Team:Warsaw/Calendar-Stage1/7 September 2010
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- | <br /><strong>legend: | + | <br> 07.09.2010 Tuesday |
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+ | <br><div class="note"> Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]</div> | ||
+ | <br>1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion | ||
+ | <br>2. Preparation of samples and examining them in flow cytometer | ||
+ | <br> | ||
+ | <br><div class="note"> Data analysis with Cell Quest[Cherry]</div> | ||
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+ | <br /><strong>legend:</strong> | ||
<hr /> | <hr /> | ||
<br />07-09-2010.004- pH 7 | <br />07-09-2010.004- pH 7 | ||
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<br />07-09-2010.007- pH 4,8 | <br />07-09-2010.007- pH 4,8 | ||
<br />07-09-2010.010- pH 8 | <br />07-09-2010.010- pH 8 | ||
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+ | <br /><br />cytometer plots: | ||
+ | <br>FSC- Forward Scatter, cell size | ||
+ | <br>SSC- Side Scatter, cell complexity | ||
+ | <br>FL-1- FL-1 sensor, cell fluorescence | ||
+ | <br /><br><hr /> | ||
<br /> | <br /> | ||
+ | Data from Cell Quest analysis: | ||
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Latest revision as of 21:58, 27 October 2010
07.09.2010 Tuesday
Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]
1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion
2. Preparation of samples and examining them in flow cytometer
Data analysis with Cell Quest[Cherry]
legend:
07-09-2010.004- pH 7
07-09-2010.005- pH 8
07-09-2010.006- pH 6,8
07-09-2010.007- pH 4,8
07-09-2010.010- pH 8
cytometer plots:
FSC- Forward Scatter, cell size
SSC- Side Scatter, cell complexity
FL-1- FL-1 sensor, cell fluorescence
Data from Cell Quest analysis:
![](https://static.igem.org/mediawiki/2010/d/db/Rbs.7.09_01.png)
![](https://static.igem.org/mediawiki/2010/8/8b/Rbs.7.09_02.png)
![](https://static.igem.org/mediawiki/2010/c/cf/Rbs.7.09_03.png)
![](https://static.igem.org/mediawiki/2010/c/c3/Rbs.7.09_04.png)
![](https://static.igem.org/mediawiki/2010/4/4d/Rbs.7.09_05.png)
![](https://static.igem.org/mediawiki/2010/6/6c/Rbs.7.09_06.png)