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Team:Warsaw/Calendar-Stage1/7 September 2010
From 2010.igem.org
07.09.2010 Tuesday
Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]
1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion
2. Preparation of samples and examining them in flow cytometer
Data analysis with Cell Quest[Cherry]
legend:
07-09-2010.004- pH 7
07-09-2010.005- pH 8
07-09-2010.006- pH 6,8
07-09-2010.007- pH 4,8
07-09-2010.010- pH 8
cytometer plots:
FSC- Forward Scatter, cell size
SSC- Side Scatter, cell complexity
FL-1- FL-1 sensor, cell fluorescence
Data from Cell Quest analysis:
