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Latest revision as of 09:46, 27 October 2010

We present our modeling details of this AHL-induced LuxR-regulated lux pR-gfp system. We assume:

There are 12 species in total:

0:E_coli: E.coli cell in flask;
1:AHL: AHL added in the flask;
2:DNA: plasmid transformed into E.coli at the beginning;
3:AHL: AHL in E.coli because of diffusion;
4:RNA: mRNA of GFP;
5:RNA: mRNA of LuxR;
6:E0040_GFP: GFP protein;
7:C0062_LuxR: LuxR protein;
8:E0040_GFP_mature: mature GFP protein;
9:AHL;C0062_LuxR: complex of AHL and LuxR;
10:(AHL)2;(C0062_LuxR)2: dimer of complex AHL-LuxR;
11:(AHL)2;(C0062_LuxR)2;DNA: binding complex of AHL-LuxR dimer and lux pR on plasmid DNA;

There are 39 reactions in total. A screenshot is provided below:

@@ Line 1: / Line 1: @@
-Environmental Health and Safety completion papers:
+{{Team:USTC_Software/Header}}
+We present our modeling details of this AHL-induced LuxR-regulated lux pR-gfp system. We assume:
+*LuxR does not form dimer;
+*AHL binds to LuxR and forms AHL-LuxR complex;
+*AHL-LuxR could form dimer, (AHL-LuxR)2;
+*Only (AHL-LuxR)2 could activate transcription from promoter lux pR;
+There are 12 species in total:
+*0:E_coli: E.coli cell in flask;
+*1:AHL: AHL added in the flask;
+*2:DNA: plasmid transformed into E.coli at the beginning;
+*3:AHL: AHL in E.coli because of diffusion;
+*4:RNA: mRNA of GFP;
+*5:RNA: mRNA of LuxR;
+*6:E0040_GFP: GFP protein;
+*7:C0062_LuxR: LuxR protein;
+*8:E0040_GFP_mature: mature GFP protein;
+*9:AHL;C0062_LuxR: complex of AHL and LuxR;
+*10:(AHL)2;(C0062_LuxR)2: dimer of complex AHL-LuxR;
+*11:(AHL)2;(C0062_LuxR)2;DNA: binding complex of AHL-LuxR dimer and lux pR on plasmid DNA;
+There are 39 reactions in total. A screenshot is provided below:
+[[Image:USTCs Reaction1.PNG|700px|center]]