Team:Peking/Notebook/Protocols

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<font size=5><b><font color=#74DF00>Protocols of Experiment Ver.2010</font></font></b>
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==Peking 2010 team==
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/SDS&WB"><font size=3><font color=#FFFFFF>*SDS-PAGE & western blot protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/7/7e/SDS-PAGE_%26_western_blot_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/SDS&W"><font size=3><font color=#FFFFFF>*SDS-PAGE & western sample preparation protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/f/f8/SDS-PAGE_%26_western_sample_preparation_protocol-xteng.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/MBP"><font size=3><font color=#FFFFFF>*Protocol for chemical inducible expression of MBP & sample preparation</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/0/08/Protocol_for_chemical_inducible_expression_of_MBP_%26_sample_preparation.pdf" target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/DDDP"><font size=3><font color=#FFFFFF>*DNA Double Digestion Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/8/84/DNA_double_digestion_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/DGEP"><font size=3><font color=#FFFFFF>*DNA Gel Extraction Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/8/83/DNA_Gel_extraction_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/MP"><font size=3><font color=#FFFFFF>*Miniprep Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/1/19/Miniprep_Protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PCIEG"><font size=3><font color=#FFFFFF>*Protocol for Chemical Inducible Expression of GFP</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/0/06/Protocol_for_chemical_inducible_expression_of_GFP.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PDPRM"><font size=3><font color=#FFFFFF>*Protocol for DNA Purification from Reaction Mixture</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/b/bf/Protocol_for_DNA_purification_from_reaction_mixture.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PLIDPVD"><font size=3><font color=#FFFFFF>*Protocol for Ligation of Insert DNA into Plasmid Vector DNA</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/0/04/Protocol_for_ligation_of_insert_DNA_into.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<font size=3><font color=#FFFFFF>*Protocol for PCR with EasyPfu DNA Polymerase Preparation for Reaction</font></font>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/d/da/Protocol_for_PCR_with_EasyPfu_DNA_Polymerase_Preparation_for_Reaction_Mixture.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PPCCT"><font size=3><font color=#FFFFFF>*Protocol for Preparation of Competent Cells for Transformation</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/4/43/Protocol_for_preparation_of_competent_cells_for_transformation.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/SDMP"><font size=3><font color=#FFFFFF>*Site-directed Mutagenesis Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/d/d0/Site_directed_mutagenesis_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/TP"><font size=3><font color=#FFFFFF>*Transformation Protocol</font></font></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://static.igem.org/mediawiki/2010/7/75/Transformation_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br>
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<br><br>
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</div>
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<div id="notebook home">
 +
<div id="titlegreen">
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<br><br>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<font size=5><b><font color=#74DF00>Protocols of Experiment Ver.2009</font></font></b>
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</div>
<div id="middlegreen">
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==Peking 2009 team==
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<p><font color=#FFFFFF>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
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<font size=4><b><font color=#FFFFFF>----Protocols Inherited from PKU IGEM 2009----</font></font></b><br><br>
 
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<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
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<a href="https://static.igem.org/mediawiki/2009/0/00/PKU_Miniprep_Protocol.pdf"><font size=3><font color=#FFFFFF>Miniprep Protocol</font></font></a>
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<a href="https://static.igem.org/mediawiki/2009/0/00/PKU_Miniprep_Protocol.pdf"target="blank"><font size=3><font color=#FFFFFF>Miniprep Protocol</font></font></a>
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<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
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<a href="https://static.igem.org/mediawiki/2009/8/85/PKU_DNA_Gel_extraction_protocol.pdf"><font size=3><font color=#FFFFFF>DNA Gel Extraction Protocol</font></font></a>
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<a href="https://static.igem.org/mediawiki/2009/8/85/PKU_DNA_Gel_extraction_protocol.pdf"target="blank"><font size=3><font color=#FFFFFF>DNA Gel Extraction Protocol</font></font></a>
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<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
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<a href="https://static.igem.org/mediawiki/2009/1/1d/PKU_DNA_digestion_protocol.pdf"><font size=3><font color=#FFFFFF>DNA Double Digestion Protocol</font></font></a>
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<a href="https://static.igem.org/mediawiki/2009/1/1d/PKU_DNA_digestion_protocol.pdf"target="blank"><font size=3><font color=#FFFFFF>DNA Double Digestion Protocol</font></font></a>
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<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
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<a href="https://static.igem.org/mediawiki/2009/4/46/PKU_Protocol_for_DNA_purification_from_reaction_mixture.pdf"><font size=3><font color=#FFFFFF>Protocol for DNA Purification From Reaction Mixture</font></font></a>
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<a href="https://static.igem.org/mediawiki/2009/4/46/PKU_Protocol_for_DNA_purification_from_reaction_mixture.pdf"target="blank"><font size=3><font color=#FFFFFF>Protocol for DNA Purification From Reaction Mixture</font></font></a>
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<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
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<a href="https://static.igem.org/mediawiki/2009/6/61/PKU_Protocol_for_ligation_of_insert_DNA_into_plasmid_vector_DNA.pdf"><font size=3><font color=#FFFFFF>Ligation of Inserting DNA into Plasmid Vector DNA</font></font></a>
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<a href="https://static.igem.org/mediawiki/2009/6/61/PKU_Protocol_for_ligation_of_insert_DNA_into_plasmid_vector_DNA.pdf"target="blank"><font size=3><font color=#FFFFFF>Ligation of Inserting DNA into Plasmid Vector DNA</font></font></a>
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
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<a href="https://static.igem.org/mediawiki/2009/d/da/PKU_Protocol_for_PCR_with_EasyPfu_DNA_Polymerase.pdf"><font size=3><font color=#FFFFFF>Protocol for PCR with EasyPfu DNA Polymerase</font></font></a>
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<a href="https://static.igem.org/mediawiki/2009/d/da/PKU_Protocol_for_PCR_with_EasyPfu_DNA_Polymerase.pdf"target="blank"><font size=3><font color=#FFFFFF>Protocol for PCR with EasyPfu DNA Polymerase</font></font></a>
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
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<a href="https://static.igem.org/mediawiki/2009/b/ba/PKU_Protocol_for_preparation_of_competent_cells_for_transformation.pdfdf"><font size=3><font color=#FFFFFF>Protocol for preparation of competent cells for transformation</font></font></a>
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<a href="https://static.igem.org/mediawiki/2009/b/ba/PKU_Protocol_for_preparation_of_competent_cells_for_transformation.pdf"target="blank"><font size=3><font color=#FFFFFF>Protocol for preparation of competent cells for transformation</font></font></a>
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
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<a href="https://static.igem.org/mediawiki/2009/6/6f/PKU_Site-directed_mutagenesis_protocol.pdf"><font size=3><font color=#FFFFFF>Site-directed mutagenesis protocol</font></font></a>
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<a href="https://static.igem.org/mediawiki/2009/6/6f/PKU_Site-directed_mutagenesis_protocol.pdf"target="blank"><font size=3><font color=#FFFFFF>Site-directed mutagenesis protocol</font></font></a>
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
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<a href="https://static.igem.org/mediawiki/2009/1/11/PKU_Transformation_protocol.pdf"><font size=3><font color=#FFFFFF>Transformation Protocol</font></font></a>
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<a href="https://static.igem.org/mediawiki/2009/1/11/PKU_Transformation_protocol.pdf"target="blank"><font size=3><font color=#FFFFFF>Transformation Protocol</font></font></a>
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>
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<a href="https://static.igem.org/mediawiki/2009/1/13/PKU_Protocol_for_chemical_inducible_expression_of_GFP.pdf"><font size=3><font color=#FFFFFF>Protocol for Chemical Inducible Expression of GFP</font></font></a>
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<a href="https://static.igem.org/mediawiki/2009/1/13/PKU_Protocol_for_chemical_inducible_expression_of_GFP.pdf"target="blank"><font size=3><font color=#FFFFFF>Protocol for Chemical Inducible Expression of GFP</font></font></a>
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Latest revision as of 05:54, 26 October 2010




   Protocols


        Notebook > Protocols


      Protocols of Experiment Ver.2010

Peking 2010 team


           *SDS-PAGE & western blot protocol         
             download PDF version

           *SDS-PAGE & western sample preparation protocol         
             download PDF version

           *Protocol for chemical inducible expression of MBP & sample preparation         
             download PDF version

           *DNA Double Digestion Protocol         
             download PDF version

           *DNA Gel Extraction Protocol         
             download PDF version

           *Miniprep Protocol         
             download PDF version

           *Protocol for Chemical Inducible Expression of GFP         
             download PDF version

           *Protocol for DNA Purification from Reaction Mixture         
             download PDF version

           *Protocol for Ligation of Insert DNA into Plasmid Vector DNA         
             download PDF version

           *Protocol for PCR with EasyPfu DNA Polymerase Preparation for Reaction         
             download PDF version

           *Protocol for Preparation of Competent Cells for Transformation         
             download PDF version

           *Site-directed Mutagenesis Protocol         
             download PDF version

           *Transformation Protocol         
             download PDF version




      Protocols of Experiment Ver.2009

Peking 2009 team


              Miniprep Protocol
              DNA Gel Extraction Protocol
              DNA Double Digestion Protocol
              Protocol for DNA Purification From Reaction Mixture
              Ligation of Inserting DNA into Plasmid Vector DNA
              Protocol for PCR with EasyPfu DNA Polymerase
              Protocol for preparation of competent cells for transformation
              Site-directed mutagenesis protocol
              Transformation Protocol
              Protocol for Chemical Inducible Expression of GFP