"
BIOTEC Dresden/Notepad/21 September 2010
From 2010.igem.org
(Difference between revisions)
| Line 17: | Line 17: | ||
The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated. | The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated. | ||
| - | |||
| - | |||
'''Fusion Protein''' | '''Fusion Protein''' | ||
| Line 24: | Line 22: | ||
Ligation of the parts was carried out followed by their transformation. | Ligation of the parts was carried out followed by their transformation. | ||
Gradient PCR for the fusion parts was done. | Gradient PCR for the fusion parts was done. | ||
| - | |||
| - | |||
| - | |||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Revision as of 14:10, 24 October 2010
Parts Assembly
PCR amplification of the following parts and the plasmid backbone containing chloramphenicol was done.
4a, 9b, 14f, 20f, 21f
The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated.
Fusion Protein
Ligation of the parts was carried out followed by their transformation. Gradient PCR for the fusion parts was done.
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
