Team:Newcastle/16 June 2010
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Swoodhouse (Talk | contribs) (New page: =PCR purification protocol= # Add 5 volumes of Buffer PBI to 1 volume of the PCR reaction and mix. If the color of the mixture is orange or violet, add 10 µl of 3 M sodium acetate, pH 5.0...) |
Shethharsh08 (Talk | contribs) (→QIAquick Gel Extraction Microcentrifuge) |
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- | =PCR purification | + | {{Team:Newcastle/mainbanner}} |
- | + | ||
- | + | =PCR purification= | |
- | + | ||
- | + | ==Aim== | |
- | + | To purify the amplified fragment from PCR by using QIAquick PCR purification kit. | |
- | + | ||
- | + | ==Materials and Protocol== | |
+ | Please refer to: [[Team:Newcastle/PCR_purification| PCR purification]] for materials required and the protocol. | ||
+ | |||
+ | =DNA ligation= | ||
+ | |||
+ | ==Aim== | ||
+ | To ligate different fragments of DNA which either has similar sticky or blunt ends. | ||
+ | |||
+ | ==Materials and Protocol== | ||
+ | Please refer to: [[Team:Newcastle/Ligation|DNA Ligation]] for materials required and the protocol. | ||
+ | |||
+ | =Transformation= | ||
+ | |||
+ | ==Aim== | ||
+ | To insert a vector or a piece of DNA into ''Bacillus subtilis''. | ||
+ | |||
+ | ==Materials and Protocol== | ||
+ | Please refer to:[[Team:Newcastle/Transformation of B. subtilis| Transformation of ''Bacillus subtilis'']] for materials required and the protocol. | ||
+ | |||
+ | =QIAquick Gel Extraction Microcentrifuge= | ||
+ | ==Aim== | ||
+ | To extract the DNA from the agarose gel by using QIAquick Gel Extraction Kit. | ||
+ | |||
+ | ==Materials and Protocol== | ||
+ | Please refer to:[[Team:Newcastle/Gel extraction| Gel extraction]] for materials required and the protocol. | ||
+ | |||
+ | {{Team:Newcastle/footer}} |
Latest revision as of 19:51, 21 October 2010
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Contents |
PCR purification
Aim
To purify the amplified fragment from PCR by using QIAquick PCR purification kit.
Materials and Protocol
Please refer to: PCR purification for materials required and the protocol.
DNA ligation
Aim
To ligate different fragments of DNA which either has similar sticky or blunt ends.
Materials and Protocol
Please refer to: DNA Ligation for materials required and the protocol.
Transformation
Aim
To insert a vector or a piece of DNA into Bacillus subtilis.
Materials and Protocol
Please refer to: Transformation of Bacillus subtilis for materials required and the protocol.
QIAquick Gel Extraction Microcentrifuge
Aim
To extract the DNA from the agarose gel by using QIAquick Gel Extraction Kit.
Materials and Protocol
Please refer to: Gel extraction for materials required and the protocol.
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