PCR purification

Aim

To purify the amplified fragment from PCR by using QIAquick PCR purification kit.

Materials and Protocol

Please refer to: PCR purification for materials required and the protocol.

DNA ligation

Aim

To ligate different fragments of DNA which either has similar sticky or blunt ends.

Materials and Protocol

Please refer to: DNA Ligation for materials required and the protocol.

Transformation

Aim

To insert a vector or a piece of DNA into Bacillus subtilis.

Materials and Protocol

Please refer to: Transformation of Bacillus subtilis for materials required and the protocol.

QIAquick Gel Extraction Microcentrifuge

Aim

To extract the DNA from the agarose gel by using QIAquick Gel Extraction Kit.

Materials and Protocol

Please refer to: Gel extraction for materials required and the protocol.