Team:KIT-Kyoto/Project

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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== The fireworks using AHL as a transmitter. ==
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The bacteria communicate with each other in the same way as people do so using some languages.This was called Quorum Sensing, and we paid our attention to this Quorum Sensing. In Quorum Sensing, the bacteria use the chemical substance called "autoinducer" in substitution for words to say with a human being. The bacteria confirm how much density the same type of bacteria exist by around oneself with autoinducers. When they sense the density is high, the bacteria take action all at once.
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We produce bioart using Quorum Sensing as materials in a colon bacillus this time. E. coli uses a chemical substance called "N-Acyl-Homoserin-Lacton(AHL)" as autoinducers.
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We thought whether we could reproduce fireworks by bioart using AHL as a communication factor.We use the GFP to express flower firelight.The GPF,which is fluorescence protein discovered by Aequorea victoria,has the property of forming a chromophoric group (chemical structure to develop a color) alone.
 
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For a rough procedure, fireworks are launched and turned off by the following methods.
 
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① GPF is expressed in E.coil using AHL as a communication factor
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② Turn off the expressed GPF like a chain reaction.
 
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|[[Image:KIT-Kyoto_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:KIT-Kyoto_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:KIT-Kyoto | Team Example]]
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:KIT-Kyoto|Home]]
 
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!align="center"|[[Team:KIT-Kyoto/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=KIT-Kyoto Official Team Profile]
 
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!align="center"|[[Team:KIT-Kyoto/Project|Project]]
 
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!align="center"|[[Team:KIT-Kyoto/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:KIT-Kyoto/Modeling|Modeling]]
 
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!align="center"|[[Team:KIT-Kyoto/Notebook|Notebook]]
 
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!align="center"|[[Team:KIT-Kyoto/Safety|Safety]]
 
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== '''Overall project''' ==
 
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Your abstract
 
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== Project Details==
 
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=== Part 2 ===
 
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=== The Experiments ===
 
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== ''E.Coli''  Pen ==
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=== Part 3 ===
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<p>
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Our team, KIT-Kyoto suggests an “''E.Coli'' Pen” as new Art Tool. This brand-new pen uses no ink but medium which ''E.Coli'' has been cultured. It is able to express more than one color with single bacterial culture. This is one of the features worthy of mention.
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''E.Coli'' Pen works with mechanism of oxidative stress reaction. Stressed by receiving H<sub>2</sub>O<sub>2</sub>, genetically modified ''E.Coli'' expresses fluorescent protein. By using some promoters with different sensitivity, each gene coding fluorescent protein at their downstream has been regulated.
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Different from previous Bioart in iGEM, “''E.Coli'' Pen” enables us to purely enjoy the Art with feeling affinity to biotechnology.
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</p>
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== Human Practice ==
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(1)Open Campus
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:On August 10,we made a booth to introduce iGEM in KIT Open Campus and explained activity of iGEM to a visitor.
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:[[Image:KITOC1.jpg|150px]] [[Image:KITOC2.jpg|150px]] [[Image:KITOC3.jpg|150px]] [[Image:KITOC4.jpg|150px]] [[Image:KITOC5.jpg|150px]]
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(2)iGEM Japan Survey
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== Results ==
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{{Template:KIT-Kyoto-1}}

Latest revision as of 13:36, 21 October 2010



E.Coli Pen

Our team, KIT-Kyoto suggests an “E.Coli Pen” as new Art Tool. This brand-new pen uses no ink but medium which E.Coli has been cultured. It is able to express more than one color with single bacterial culture. This is one of the features worthy of mention. E.Coli Pen works with mechanism of oxidative stress reaction. Stressed by receiving H2O2, genetically modified E.Coli expresses fluorescent protein. By using some promoters with different sensitivity, each gene coding fluorescent protein at their downstream has been regulated. Different from previous Bioart in iGEM, “E.Coli Pen” enables us to purely enjoy the Art with feeling affinity to biotechnology.

Human Practice

(1)Open Campus

On August 10,we made a booth to introduce iGEM in KIT Open Campus and explained activity of iGEM to a visitor.
KITOC1.jpg KITOC2.jpg KITOC3.jpg KITOC4.jpg KITOC5.jpg

(2)iGEM Japan Survey



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