Team:Macquarie Australia/Protocols and Other Methods

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<h1> BioLine Isolate Genomic DNA Mini Kit: </h1>
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<ol>
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<li> Pellet cell culture, decant, add 1mL CLS-TC </li>
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<li>Resuspend and transfer to Fastprep tube (BIO101) </li>
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<li>Fastprep 5.5g for 30 seconds, then spin @ 14,000 rpm for 5 minutes </li>
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<li>Recover 700uL then 700uL Binding Matrix</li>
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<li>Rotate for 5 minutes then spin for 1 minute</li>
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<li>Decant the supernatant and add 800uL SEWS then vortex</li>
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<li>Rotate for 5 minutes then spin for 1 minute</li>
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<li>Decant the supernatant then pulse spin and remove the last bit of ethanol</li>
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<li>Air dry for 5 minutes then resuspend in 200uL TE buffer</li>
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<li>Spin at 14,000 rpm for 3 minutes and recover 160uL </li> </ol></p>
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See the BioLine manual for full protocol:
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http://www.bioline.com/pdf/guides/Nucleic%20Acid%20Isolation%20Guide.pdf

Revision as of 09:19, 19 October 2010

Protocols and Other Methods Used

BioLine Isolate Genomic DNA Mini Kit:

  1. Pellet cell culture, decant, add 1mL CLS-TC
  2. Resuspend and transfer to Fastprep tube (BIO101)
  3. Fastprep 5.5g for 30 seconds, then spin @ 14,000 rpm for 5 minutes
  4. Recover 700uL then 700uL Binding Matrix
  5. Rotate for 5 minutes then spin for 1 minute
  6. Decant the supernatant and add 800uL SEWS then vortex
  7. Rotate for 5 minutes then spin for 1 minute
  8. Decant the supernatant then pulse spin and remove the last bit of ethanol
  9. Air dry for 5 minutes then resuspend in 200uL TE buffer
  10. Spin at 14,000 rpm for 3 minutes and recover 160uL

See the BioLine manual for full protocol: http://www.bioline.com/pdf/guides/Nucleic%20Acid%20Isolation%20Guide.pdf

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