Team:Tokyo Tech/Project/wolf coli


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iGEM Tokyo Tech 2010 "E.coli with Humanity"

4 Wolf coli. overview

Figure3-1. Introduction of “Wolfcoli”

Regulation of Artificial Cooperation System (ACS)lead to alternative behavioral of E. coli.Our team challenged to design “Wolfcoli”, an E coli. that its behavior changes due to the intensity of lightinspired by famous legend of wolfman. When there is intermediate light intensity, two cells doesn’t help each other at only full moon night .  From these behaviors, we call this “Wolfcoli” (Fig. 3-1).

Figure3-2. Overview of “Wolfcoli” system

In order to create the “Wolf coli.”, we introduced " red-light-dependent gene expression network"[1] and "band-detect network"[2] and combined these networks with the Artificial Cooperation System (Fig. 3-2). We conducted experiment on series of OmpC promoter (PompC), and LacIM1 which are crucial parts of our networks.