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Contents 1 2010/9/20 Monday (watachin) 1.1 Experiment:Insert check Electrophoresis 1.2 Experiment:PCR 1.3 Experiment:Plating 1.4 Experiment:Electrophoresis 1.5 Experiment:PCR 1.6 Experiment:Digestion 1.7 Experiment:Electrophoresis 1.8 Experiment:DNA purification from agarose gel with QIAGEN 1.9 Experiment:Ligation 1.10 Experiment:Transformation

2010/9/20 Monday (watachin)

Experiment:Insert check Electrophoresis

Member
naoto and watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

Experiment:PCR

Member
naoto and watachin

Materials and Procedure

Direct PCR of bcs genes

follow the protocol(Direct PCR)

Experiment:Plating

Member
naoto and watachin

Materials and Procedure

plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth

Experiment:Electrophoresis

Member
naoto and watachin

Result Failure (I had the wrong protocol.)

Experiment:PCR

Member
naoto

Materials and Procedure

PCR of bcs genes of A.xylinum and E.coli

follow the protocol(Direct_PCR)

Experiment:Digestion

Member
watachin

Materials and Procedure

follow the protocol(Digestion)

Experiment:Electrophoresis

Member
watachin

Materials and Procedure

follow the protocol(Electrophoresis)

Result

Experiment:DNA purification from agarose gel with QIAGEN

Member
watachin

Materials and Procedure

follow the protocol(DNA purification from agarose gel with QIAGEN)

Experiment:Ligation

Member
watachin

Materials and Procedure

follow the protocol(Ligation)

Experiment:Transformation

Member
watachin

Materials and Procedure

follow the protocol(Transformation)