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	==2010/9/8 Monday (NEX)==		==2010/9/8 Monday (NEX)==
	===Experiment:digestion of bcsA,B,C(from''E.coli'')===		===Experiment:digestion of bcsA,B,C(from''E.coli'')===

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Revision as of 03:39, 16 September 2010

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2010/9/8 Monday (NEX)

Experiment:digestion of bcsA,B,C(fromE.coli)

member
bambi75 and NEX

material

• bcsA,B,C(from E.coli)
• 10×Mbuffer
• BSA
• XbaI
• SpeI

procedure

1. add "material" to PCR tubes(show below)
2. incubation at (37℃,7h)

composition

	bcsA	bcsB	bcsC
solution of bcs(μl)	60	60	60
10×Mbuffer(μl)	6	6	6
BSA(μl)		2	2
XbaI(μl)		1.2	1.2
SpeI(ul)	1.2	1.2

Experiment:Make Agarose Gels Electro-phoresis

Member
Same above

Materials

• 1×TaE 200ml
• Agarose 2g
• Ethidium bromide　10µl

Procedure

1. Add and mixture above materials in an Erlenmeyer flask
2. Warm up them in a Microwave oven