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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/30
From 2010.igem.org
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'''result'''<br/> | '''result'''<br/> | ||
| - | bands of bcsC were appeared | + | bands of bcsC were appeared<br/> |
| + | [[Image:201008301.jpg]] | ||
===Experiment:separation of ''A.xylinus''=== | ===Experiment:separation of ''A.xylinus''=== | ||
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'''result'''<br/> | '''result'''<br/> | ||
a band of bcsA were appeared | a band of bcsA were appeared | ||
| - | (below bands appreared to be primer) | + | (below bands appreared to be primer)<br/> |
| + | [[Image:201008302.jpg]] | ||
===Experiment:purification of agarose gel=== | ===Experiment:purification of agarose gel=== | ||
Revision as of 01:25, 12 September 2010
Contents |
2010/8/30 Monday (Naoto)
Experiment:electrophoresis
member
NEX,bambi75 and watachin
material
- PCR production (bcsC from E.coli)
- 1×TAE buffer
- agarose gel
procedure
follow to protocol4
result
bands of bcsC were appeared
Experiment:separation of A.xylinus
member
naoto
material
- A.xylinus in open wet ware media(8/25 made)
procedure
- take 0.5ml of A.xylinus in open wet ware media to tubes
- centrifuge 15000rpm/5min
Experiment:direct PCR
member
naoto
material
- A.xylinus separated above experiment
procedure
follow to protocol3
Experiment:purification of agarose gel
member
NEX,bambi75 and watachin
material
- bcsC from E.coli in agarose gel
- QIAGEN
procedure
follow to protocol5
Experiment:electrophoresis
member
naoto
material
- PCR production (bcsA from A.xylinus)
- 1×TAE buffer
- agarose gel
procedure
follow to protocol4
result
a band of bcsA were appeared
(below bands appreared to be primer)
Experiment:purification of agarose gel
member
naoto
material
- bcsA from A.xylinus in agarose gel
- QIAGEN
procedure
follow to protocol5
