Team:Peking/Notebook/YHu

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   Yang Hu's Notes
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For the bench work, I was responsible for the construction of mercury metal binding peptide cytosol expression module and T7 promoter drove MerR generation module. I also helped to conduct western blotting assay.On the other hand, I take charge of the work of Human Practice concerning about the potential problems of horizontal gene transfer.


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Contents

June

6.30

1. Dissolve primers: primers for mbp fragments (standardization): SD_ForA, SD_ForC, SD_Rev. primers for mbp fragments (commercialization): NdeI_A(For), NdeI_C(For), XhoI(Rev).

2. Miniprep: plasmids provided by Anne O Summers and I-120.

3. PCR reaction for mbp fragments using dissolved primers.

5x PHF buffer II 4uL

ddNTP Mixture 1.6uL

For 1uL

Rev 1uL

Template plasmid 0.5uL

phusionHF 0.2uL

ddH2O up to 20 uL

4. Electrophoresis PCR reaction system in 1% agarose gel.

5. Excise the gel slice and extract the mbp fragments.