PROJECT LAB BOOK
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DAY-BY-DAY PROGRESS FOR THE TRANSFORMATION AND CLONING
22nd September 2010
Plasmid Prep using Qiagen Plasmid Midi Prep Kit
The pET-3A vector plasmid that is to be used for the transformation needs to be purified
The pET-3A plasmid was purified using the Qiagen Plasmid Midi Prep Kit as per the manufacturer’s protocols
The only difference made to the protocol is that only 5ml of Buffer QC was used on the 2nd wash step
The plasmid (two samples A & B) as well as some column flow-through collected at different stages of the purification were run on a GelRed post-stained 1.5% agarose gel and photo taken for visualization (see below).
A NanoDrop spectrophotometer reading was also recorded to check the quality of the extracted genomic DNA.
There was no DNA pellet formed at the end of this protocol so we may have lost some DNA
There was also some plasmid observed in some of the column flow through so some plasmid has been lost
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