Team:Macquarie Australia/Notebook

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PROJECT LAB BOOK

Welcome to the Macquarie University project lab book page!

Here you will find a day-by-day account of our triumphs and failures.

A day-by-day progress for Agrobacterium Tumefaciens Bacteriophytochrome

20th August 2010

Genomic DNA extraction

  • A. tumefaciens genomic DNA extracted using the Invitrogen PureLinkTM Genomic DNA Purification kit as per the manufacturer’s protocols.

  • The extractions were run on a GelRed stained 1% agarose gel and photo taken for visualization (see below).

  • A NanoDrop spectrophotometer reading was also recorded to check the quality of the extracted genomic DNA.

  • The extraction was successful for all A. tumefaciens cell lysate samples (labeled DNA1.1, DNA1.2, DNA2 (See figure below)

    • A. tumefaciens genomic DNA extraction agarose results:

    All four DNA samples show a smear of gDNA. Because the samples were treated with RNase there is no band indicative of RNA visible = SUCCESS!

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