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Team:Macquarie Australia/Notebook
From 2010.igem.org
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| + | <h4> <center>***** Results of A. tumefaciens genomic DNA extraction ***</h4></center></big> <p> | ||
| + | <b>Figure 1. </b> GelRed stained 1% agarose gel of genomic DNA extraction from A. tumefaciens.<p> | ||
| + | In lanes 1 and 11 there is a 1kb ladder. In lane 2 is the DNA1.1 flow through, lane 3 is the DNA2.2 flow through, lane 4 is the DNA2.1 flow through and lane 4 in the DNA2.2 flowthrough. All four samples show a smear that is indicative of genomic DNA. <u> The extraction has been successful. </u> | ||
| + | <h4>Nanodrop absorbance readings: </h4> | ||
| - | + | <div align="center"> | |
| + | <table> | ||
| + | <tr> | ||
| + | <th>Genomic DNA sample</th> | ||
| + | <th>260/280 OD ratio</th> | ||
| + | <th>Concentration (ng/mL)</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>DNA1.1</td> | ||
| + | <td>1.88</td> | ||
| + | <td>351.5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>DNA1.2</td> | ||
| + | <td>1.98</td> | ||
| + | <td>203.1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>DNA2.1</td> | ||
| + | <td>2.14</td> | ||
| + | <td>738.9</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>DNA2.2</td> | ||
| + | <td>2.16</td> | ||
| + | <td>709.7</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | </div> | ||
Revision as of 01:02, 14 October 2010
PROJECT LAB BOOK
Welcome to the Macquarie University project lab book page!
Here you will find a day-by-day account of our triumphs and failures.
A day-by-day progress for Agrobacterium Tumefaciens Bacteriophytochrome
20th August 2010
Genomic DNA extraction
