Team:Macquarie Australia/Notebook

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<h2><font color="#47484c">Notebook</font></h2>
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<h1><font color="#47484c">PROJECT LAB BOOK <p>
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Welcome to the Macquarie University project lab book page! <p>
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Here you will find a day-by-day account of our triumphs and failures.</font></h1> </hr>
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<center><h2> A day-by-day progress for <i> Agrobacterium Tumefaciens </i> Bacteriophytochrome </h2>  </center>
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20th August 2010 <p>
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Genomic DNA extraction <p>
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<i> A. tumefaciens </i> genomic DNA extracted using the Invitrogen PureLinkTM Genomic DNA Purification kit as per the manufacturer’s protocols. </li> <li type="disc"> <p>
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The extractions were run on a GelRed stained 1% agarose gel and photo taken for visualization (see below). </li> <p> <li type="disc">
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A NanoDrop spectrophotometer reading was also recorded to check the quality of the extracted genomic DNA. <p> </li> <li type="disc">
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The extraction was successful for all A. tumefaciens cell lysate samples (labeled DNA1.1, DNA1.2, DNA2 (See figure below) <p> </li>
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<p><h3><i><b>A. tumefaciens </i> genomic DNA extraction agarose results: <p> </b> </h3>
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All four DNA samples show a smear of gDNA. Because the samples were treated with RNase there is no band indicative of RNA visible = SUCCESS! <p>
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
 
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Revision as of 00:50, 14 October 2010

PROJECT LAB BOOK

Welcome to the Macquarie University project lab book page!

Here you will find a day-by-day account of our triumphs and failures.

A day-by-day progress for Agrobacterium Tumefaciens Bacteriophytochrome

20th August 2010

Genomic DNA extraction

  • A. tumefaciens genomic DNA extracted using the Invitrogen PureLinkTM Genomic DNA Purification kit as per the manufacturer’s protocols.

  • The extractions were run on a GelRed stained 1% agarose gel and photo taken for visualization (see below).

  • A NanoDrop spectrophotometer reading was also recorded to check the quality of the extracted genomic DNA.

  • The extraction was successful for all A. tumefaciens cell lysate samples (labeled DNA1.1, DNA1.2, DNA2 (See figure below)

    • A. tumefaciens genomic DNA extraction agarose results:

    All four DNA samples show a smear of gDNA. Because the samples were treated with RNase there is no band indicative of RNA visible = SUCCESS!

    Retrieved from "http://2010.igem.org/Team:Macquarie_Australia/Notebook"