Team:Caltech/Week 4

Revision as of 00:57, 8 July 2010 by Lucash (Talk | contribs)

iGEM 2010

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support

Monday 7/5

Note: Today was another Institute Holiday.

Hydrolyzed linseed oil. The reaction solution will be characterized on Wednesday.

Attempted to make [http://partsregistry.org/Part:BBa_V1012 V1012] (strain CP919, KanR, <math>\Delta</math>envZ) electrocompetent:
- Centrifuged overnight 3mL LB-Kan culture at 4C, 16000rcf for 10min.
- Discarded supernatant. Resuspended pellet in 1.5mL ice-cold water. Centrifuged again.
- Repeated with 0.75mL ice-cold water.
- Resuspended pellet in 1.5mL ice-cold 10% glycerol.
- Flash-froze 100uL aliquots in dry ice/ethanol.
Transformed light/lysis ligation product from last week into [http://partsregistry.org/Part:BBa_V1012 V1012] via electroporation. (Voltage: 2.5V; time constant: 4.5)
- Plated 100uL on LB-Tet agar plate & inoculated a 5mL LB-Tet overnight culture.
Inoculated 5mL LB-Kan culture of purely [http://partsregistry.org/Part:BBa_V1012 V1012] in case we need to retry the competence procedure.

Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found