Team:Caltech/Protocols

From 2010.igem.org

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* Growing cells with PHA (and substrate oil)
* Growing cells with PHA (and substrate oil)
* [[Team:Caltech/Colony PCR|Colony PCR]]
* [[Team:Caltech/Colony PCR|Colony PCR]]
 +
* [[Team:Caltech/Cloning|Two-day Cloning]]
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Revision as of 00:00, 15 October 2010


iGEM 2010



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  • Competent Cells
  • Transformation by Electroporation
  • Making stock solutions
  • Freezer (glycerol) stocks
  • Plating antibiotics
  • Sequencing primers
  • Making synthase casette
    • Sources of sequences
    • Optimizing & checking genes
  • Chemical/Crosslinking reactions
    • Digesting substrate (hydrolysis) + epoxidation (MCPBA)
    • crosslinking (AIBN3) + UV initiation
  • Inducing lysis via 632nm light initiation
  • Making cell/glycerol/agar mixture
    • Applying this to plates
  • Growing cells with PHA (and substrate oil)
  • Colony PCR
  • Two-day Cloning
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