BIOTEC Dresden/Notepad/27 August 2010
From 2010.igem.org
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All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. | All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. | ||
+ | The plasmids pETMM43 and pETMMZZ were double digested and purified from agarose gel. | ||
<hr> | <hr> |
Revision as of 14:15, 2 September 2010
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Fusion Protein
All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. The plasmids pETMM43 and pETMMZZ were double digested and purified from agarose gel.
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