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BIOTEC Dresden/Notepad/23 July 2010
From 2010.igem.org
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For the PCR we used the [http://openwetware.org/wiki/PrbbBB:colony_pcr_v1 PCR protocol]. | For the PCR we used the [http://openwetware.org/wiki/PrbbBB:colony_pcr_v1 PCR protocol]. | ||
For the gel electrophoresis we use 1% and 2% agarose gels with [http://openwetware.org/wiki/TBE TBE buffer]. | For the gel electrophoresis we use 1% and 2% agarose gels with [http://openwetware.org/wiki/TBE TBE buffer]. | ||
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{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Revision as of 13:30, 24 October 2010
We picked two colonies for each the following parts
2a, 3a, 3b, 6a, 6b, 9a, 9b, 11a, 11b, 14a, 14b, 15a, 15b, 20a, 20b, 21a, 21b, 27a, 27b, 28a, 28b, 29a, 29b, 30a, 30b, 31a, 33a, 33b
The gel electrophoresis of the colony PCRs didn't run as expected. We have to repeat it on Monday. We decided anyhow the purify the plasmids tomorrow.
For the PCR we used the PCR protocol. For the gel electrophoresis we use 1% and 2% agarose gels with TBE buffer.
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
