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BIOTEC Dresden/Notepad/23 July 2010
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We picked two colonies for each the following parts | We picked two colonies for each the following parts | ||
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We decided anyhow the purify the plasmids tomorrow. | We decided anyhow the purify the plasmids tomorrow. | ||
| - | For the PCR we used the [ | + | For the PCR we used the [http://openwetware.org/wiki/PrbbBB:colony_pcr_v1 PCR protocol]. |
For the gel electrophoresis we use 1% and 2% agarose gels with [http://openwetware.org/wiki/TBE TBE buffer]. | For the gel electrophoresis we use 1% and 2% agarose gels with [http://openwetware.org/wiki/TBE TBE buffer]. | ||
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Latest revision as of 21:32, 27 October 2010
We picked two colonies for each the following parts
2a, 3a, 3b, 6a, 6b, 9a, 9b, 11a, 11b, 14a, 14b, 15a, 15b, 20a, 20b, 21a, 21b, 27a, 27b, 28a, 28b, 29a, 29b, 30a, 30b, 31a, 33a, 33b
The gel electrophoresis of the colony PCRs didn't run as expected. We have to repeat it on Monday. We decided anyhow the purify the plasmids tomorrow.
For the PCR we used the PCR protocol. For the gel electrophoresis we use 1% and 2% agarose gels with TBE buffer.
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
