BIOTEC Dresden/Notepad/21 September 2010

(Difference between revisions)

Revision as of 15:04, 22 October 2010

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Parts Assembly

PCR amplification of the following parts and the plasmid backbone containing chloramphenicol was done.

4a, 9b, 14f, 20f, 21f

The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated.

Fusion Protein

Ligation of the parts was carried out followed by their transformation. Gradient PCR for the fusion parts was done.

@@ Line 22: / Line 22: @@
 '''Fusion Protein'''
-A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.
+Ligation of the parts was carried out followed by their transformation.
+Gradient PCR for the fusion parts was done.
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