BIOTEC Dresden/Notepad/21 September 2010

From 2010.igem.org

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The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated.
The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated.
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'''Fusion Protein'''
'''Fusion Protein'''
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A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.  
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Ligation of the parts was carried out followed by their transformation.
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Gradient PCR for the fusion parts was done.
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<hr>
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[[Category:BIOTEC Dresden/Notepad/September|September]]
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{{Biotec_Dresden/month}}
{{Biotec_Dresden/month}}
{{Biotec_Dresden/Bottom}}
{{Biotec_Dresden/Bottom}}

Latest revision as of 22:48, 27 October 2010

Parts Assembly

PCR amplification of the following parts and the plasmid backbone containing chloramphenicol was done.

4a, 9b, 14f, 20f, 21f

The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated.

Fusion Protein

Ligation of the parts was carried out followed by their transformation. Gradient PCR for the fusion parts was done.


July
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August
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September
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October
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