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BIOTEC Dresden/Notepad/16 August 2010
From 2010.igem.org
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'''AHL assay''' | '''AHL assay''' | ||
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An AHL assay with part 24 (BBA_T9002) was carried in a 96 well plate with a step of 150 nM AHL concentration (range 150 - 1500 nM). | An AHL assay with part 24 (BBA_T9002) was carried in a 96 well plate with a step of 150 nM AHL concentration (range 150 - 1500 nM). | ||
Lb was used for blank, in addition pure bacterial culture (OD=6) was used as control. | Lb was used for blank, in addition pure bacterial culture (OD=6) was used as control. | ||
Reading done at an emission wavelength of 535 nm and excitation wavelength 485 nm | Reading done at an emission wavelength of 535 nm and excitation wavelength 485 nm | ||
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However, a different amount of bacterial culture was used for wells of different AHL concentration, a necessary improvement for the next detection will be to introduce an equal nr of cells in each well and adjust the volume difference with LB | However, a different amount of bacterial culture was used for wells of different AHL concentration, a necessary improvement for the next detection will be to introduce an equal nr of cells in each well and adjust the volume difference with LB | ||
| - | + | '''Fusion Protein''' | |
| + | A restriction digest on the purified plasmids was carried out to confirm if the ligation worked and the insert has the correct size. | ||
| + | [[Category:BIOTEC Dresden/Notepad/August|August]] | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 21:58, 27 October 2010
AHL assay
An AHL assay with part 24 (BBA_T9002) was carried in a 96 well plate with a step of 150 nM AHL concentration (range 150 - 1500 nM). Lb was used for blank, in addition pure bacterial culture (OD=6) was used as control.
Reading done at an emission wavelength of 535 nm and excitation wavelength 485 nm
However, a different amount of bacterial culture was used for wells of different AHL concentration, a necessary improvement for the next detection will be to introduce an equal nr of cells in each well and adjust the volume difference with LB
Fusion Protein
A restriction digest on the purified plasmids was carried out to confirm if the ligation worked and the insert has the correct size.
July |
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August |
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September |
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October |
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