TeamNewcastleNanoDrop Spectrophotometer
From 2010.igem.org
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# Log onto computer and select Nanodrop program from the desktop (ND 1000) | # Log onto computer and select Nanodrop program from the desktop (ND 1000) | ||
- | # | + | # Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean. |
- | # | + | # After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (Example: Miniprep samples should be equalized with EB buffer). |
+ | # Use DNA-50 for DNA samples. | ||
# Wipe to remove buffer and apply 3 μl of sample to nib. Press measure. | # Wipe to remove buffer and apply 3 μl of sample to nib. Press measure. | ||
# If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples) | # If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples) | ||
# After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off. | # After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off. | ||
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{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Revision as of 14:45, 29 July 2010
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NanoDrop Spectrophotometer
Nanodrop can be used to measure the DNA, RNA and protein.
Measure the concentration and purity of extracted DNA using absorbance (using the automated nanodrop machine!)
The ideal concentration of DNA is 150 ng/ml!
Procedures
- Log onto computer and select Nanodrop program from the desktop (ND 1000)
- Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.
- After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (Example: Miniprep samples should be equalized with EB buffer).
- Use DNA-50 for DNA samples.
- Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.
- If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
- After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.