Team:Warsaw/Calendar-Stage1/1 July 2010

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Marcin

Task 1: Prepare bacterial culture to isolate BBa_K177044 from pSB1A3. Task 2: Another ligation of [http://partsregistry.org/Part:BBa_K177044BBa_K177044] Methods: * Ligation mixtures composition: 
10 μl digested insert
8 μl digested vector 
5 μl Tango buffer(Fermentas)
2 μl ATP 10 mM solution 
4 μl MQ water
1 μl ligase T4 (Fermentas)
* Ligation was carried out about 20 hours and subsequently inactivated

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