Team:Warsaw/Calendar-Stage1/1 July 2010

• Team
• Attribution
• Sponsors
• Acknowledgemets

• Idea
• Background
• Promoters Measured
• RBSes Measured
• ExpressIt! Vectors
• Modeling

• Idea
• Background
• Design
• Results

• Idea
• Background and Design
• Results
• Gallery of microphotographs

• Idea
• Background
• Design
• Results
• Modeling

• Parts
• Labbook

• Licensing of biological parts
• Syntetic biology in Poland
• Synthetic BioLectures gallery
• iGEM survey

• About Biobrick Manager
• See Biobrick Manager

1.07.2010 Thursday


Use a protocol for competent cells preparation to get competent Escherichia coli TOP10 strain.


Biobricks from 2010 distribution: GFP-terminator, J61100 J61101 J61107 J61117 J61127 [Anderson's RBSes], B0030 B0031 B0032 B0033 B0034 [community RBSes], J23100 [synthetic promoter].
1. Distributed in 10 ul of TE [skład].
2. 5 ul of DNA used for transformation [according to protocol].


1. Alkaline lysis no1 [according to protocol].
2. Restriction digest
3. Run agarose gel