Team:Warsaw/Calendar-Stage1/1 July 2010

From 2010.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 11: Line 11:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<!-- do not edit above me! -->
 +
<!-- div class note gives the strip with the light brown gackground, it is declared in the css for our webpage! -->
 +
 +
<br> 1.07.2010 Thursday
 +
<br>
 +
<br><div class="note"> Previous day: Preparation of competent cells [Ania P., Jarek]</div>
 +
<br> Use a protocol for competent cells preparation to get competent Escherichia coli TOP10 strain.
 +
<br>
 +
 +
<br><div class="note"> Previous day: Revitalisation of biobricks [Ania P., Jarek]</div>
 +
<br> Biobricks from 2010 distribution:
 +
GFP-terminator,
 +
<a href="http://partsregistry.org/Part:BBa_J61100">J61100</a>
 +
<a href="http://partsregistry.org/Part:BBa_J61101">J61101</a>
 +
<a href="http://partsregistry.org/Part:BBa_J61107">J61107</a>
 +
<a href="http://partsregistry.org/Part:BBa_J61117">J61117</a>
 +
<a href="http://partsregistry.org/Part:BBa_J61127">J61127</a>
 +
[Anderson's RBSes],
 +
<a href="http://partsregistry.org/Part:BBa_B0030">B0030</a>
 +
<a href="http://partsregistry.org/Part:BBa_B0031">B0031</a>
 +
<a href="http://partsregistry.org/Part:BBa_B0032">B0032</a>
 +
<a href="http://partsregistry.org/Part:BBa_B0033">B0033</a>
 +
<a href="http://partsregistry.org/Part:BBa_B0034">B0034</a>
 +
[community RBSes],
 +
<a href="http://partsregistry.org/Part:BBa_J23100">J23100</a>
 +
[synthetic promoter].
 +
<br> 1. Distributed in 10 ul of TE [skład].
 +
<br> 2. 5 ul of DNA used for transformation [according to protocol].
 +
<br>
 +
 +
<br><div class="note"> Checking correctness of biobricks [Ania P., Jarek]</div>
 +
<br> 1. Alkaline lysis no1 [according to protocol].
 +
<br> 2. Restriction digest
 +
<br> 3. Run agarose gel
 +
<br>
 +
<!-- use br to go to the next line also to ctrate empty lines! -->

Latest revision as of 13:17, 31 July 2010

Example Tabs


1.07.2010 Thursday

Previous day: Preparation of competent cells [Ania P., Jarek]

Use a protocol for competent cells preparation to get competent Escherichia coli TOP10 strain.

Previous day: Revitalisation of biobricks [Ania P., Jarek]

Biobricks from 2010 distribution: GFP-terminator, J61100 J61101 J61107 J61117 J61127 [Anderson's RBSes], B0030 B0031 B0032 B0033 B0034 [community RBSes], J23100 [synthetic promoter].
1. Distributed in 10 ul of TE [skład].
2. 5 ul of DNA used for transformation [according to protocol].

Checking correctness of biobricks [Ania P., Jarek]

1. Alkaline lysis no1 [according to protocol].
2. Restriction digest
3. Run agarose gel

Retrieved from "http://2010.igem.org/Team:Warsaw/Calendar-Stage1/1_July_2010"