Team:Warsaw/Calendar-Stage1/1 July 2010

From 2010.igem.org

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<h3><div style="text-align: center;">Main topic</div></h3>
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<h4>Marcin</h4>
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Task 1: Prepare bacterial culture to isolate <a href="http://partsregistry.org/Part:BBa_K177044" style="color: black">BBa_K177044</a> from <a href="http://partsregistry.org/Part:pSB1A3 style="color:black">pSB1A3</a>.
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Task 2: Another ligation of [http://partsregistry.org/Part:BBa_K177044<span style="color: black">BBa_K177044</span>]
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<br> 1.07.2010 Thursday
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<br>
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<br><div class="note"> Previous day: Preparation of competent cells [Ania P., Jarek]</div>
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<br> Use a protocol for competent cells preparation to get competent Escherichia coli TOP10 strain.
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<br>
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Methods:
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<br><div class="note"> Previous day: Revitalisation of biobricks [Ania P., Jarek]</div>
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* Ligation mixtures composition:
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<br> Biobricks from 2010 distribution:  
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<pre>
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GFP-terminator,
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10 μl digested insert
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<a href="http://partsregistry.org/Part:BBa_J61100">J61100</a>
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8 μl digested vector
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<a href="http://partsregistry.org/Part:BBa_J61101">J61101</a>
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5 μl Tango buffer(Fermentas)
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<a href="http://partsregistry.org/Part:BBa_J61107">J61107</a>
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2 μl ATP 10 mM solution
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<a href="http://partsregistry.org/Part:BBa_J61117">J61117</a>
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4 μl MQ water
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<a href="http://partsregistry.org/Part:BBa_J61127">J61127</a>
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1 μl ligase T4 (Fermentas)
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[Anderson's RBSes],
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</pre>      
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<a href="http://partsregistry.org/Part:BBa_B0030">B0030</a>
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* Ligation was carried out about 20 hours and subsequently inactivated
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<a href="http://partsregistry.org/Part:BBa_B0031">B0031</a>
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<a href="http://partsregistry.org/Part:BBa_B0032">B0032</a>
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<a href="http://partsregistry.org/Part:BBa_B0033">B0033</a>
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<a href="http://partsregistry.org/Part:BBa_B0034">B0034</a>
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[community RBSes],
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<a href="http://partsregistry.org/Part:BBa_J23100">J23100</a>
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[synthetic promoter].
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<br> 1. Distributed in 10 ul of TE [skład].
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<br> 2. 5 ul of DNA used for transformation [according to protocol].
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<br>
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<br><div class="note"> Checking correctness of biobricks [Ania P., Jarek]</div>
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<br> 1. Alkaline lysis no1 [according to protocol].
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<br> 2. Restriction digest
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<br> 3. Run agarose gel
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<br>
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Latest revision as of 13:17, 31 July 2010

Example Tabs


1.07.2010 Thursday

Previous day: Preparation of competent cells [Ania P., Jarek]

Use a protocol for competent cells preparation to get competent Escherichia coli TOP10 strain.

Previous day: Revitalisation of biobricks [Ania P., Jarek]

Biobricks from 2010 distribution: GFP-terminator, J61100 J61101 J61107 J61117 J61127 [Anderson's RBSes], B0030 B0031 B0032 B0033 B0034 [community RBSes], J23100 [synthetic promoter].
1. Distributed in 10 ul of TE [skład].
2. 5 ul of DNA used for transformation [according to protocol].

Checking correctness of biobricks [Ania P., Jarek]

1. Alkaline lysis no1 [according to protocol].
2. Restriction digest
3. Run agarose gel

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