Team:Warsaw/Calendar-Stage1/1 July 2010

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<h3><div style="text-align: center;">Main topic</div></h3>
 
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<h4>Marcin</h4>
 
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<br>
 
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Task 1: Prepare bacterial culture to isolate <a href="http://partsregistry.org/Part:BBa_K177044" style="color: black">BBa_K177044</a> from <a href="http://partsregistry.org/Part:pSB1A3 style="color:black">pSB1A3</a>.
 
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<br>
 
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Task 2: Another ligation of  <a href="http://partsregistry.org/Part:BBa_K177044<span style="color: black">BBa_K177044</a>
 
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<br>
 
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Methods:
 
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* Ligation mixtures composition:
 
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<pre style="font-size:100%">
 
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10 μl digested insert
 
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8 μl digested vector
 
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5 μl Tango buffer(Fermentas)
 
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2 μl ATP 10 mM solution
 
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4 μl MQ water
 
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1 μl ligase T4 (Fermentas)
 
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</pre>     
 
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* Ligation was carried out about 20 hours and subsequently inactivated
 

Revision as of 22:33, 9 July 2010

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