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Team:Warsaw/Calendar-Stage1/1 July 2010
From 2010.igem.org
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Methods: | Methods: | ||
* Ligation mixtures composition: | * Ligation mixtures composition: | ||
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10 μl digested insert | 10 μl digested insert | ||
8 μl digested vector | 8 μl digested vector | ||
Revision as of 21:25, 9 July 2010
Main topic
Marcin
Task 1: Prepare bacterial culture to isolate BBa_K177044 from pSB1A3.
Task 2: Another ligation of BBa_K177044
Methods: * Ligation mixtures composition:
10 μl digested insert 8 μl digested vector 5 μl Tango buffer(Fermentas) 2 μl ATP 10 mM solution 4 μl MQ water 1 μl ligase T4 (Fermentas)* Ligation was carried out about 20 hours and subsequently inactivated
