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Team:Warsaw/Calendar-Stage1/1 July 2010
From 2010.igem.org
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<h3><div style="text-align: center;">Main topic</div></h3> | <h3><div style="text-align: center;">Main topic</div></h3> | ||
<h4>Marcin</h4> | <h4>Marcin</h4> | ||
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Task 1: Prepare bacterial culture to isolate <a href="http://partsregistry.org/Part:BBa_K177044" style="color: black">BBa_K177044</a> from <a href="http://partsregistry.org/Part:pSB1A3 style="color:black">pSB1A3</a>. | Task 1: Prepare bacterial culture to isolate <a href="http://partsregistry.org/Part:BBa_K177044" style="color: black">BBa_K177044</a> from <a href="http://partsregistry.org/Part:pSB1A3 style="color:black">pSB1A3</a>. | ||
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| - | Task 2: Another ligation of | + | Task 2: Another ligation of <a href="http://partsregistry.org/Part:BBa_K177044<span style="color: black">BBa_K177044</a> |
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Methods: | Methods: | ||
* Ligation mixtures composition: | * Ligation mixtures composition: | ||
Revision as of 21:23, 9 July 2010
Main topic
Marcin
Task 1: Prepare bacterial culture to isolate BBa_K177044 from pSB1A3.
Task 2: Another ligation of BBa_K177044
Methods: * Ligation mixtures composition:
10 μl digested insert 8 μl digested vector 5 μl Tango buffer(Fermentas) 2 μl ATP 10 mM solution 4 μl MQ water 1 μl ligase T4 (Fermentas)* Ligation was carried out about 20 hours and subsequently inactivated
