"
Team:Warsaw/Calendar-Stage1/1 July 2010
From 2010.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
<h4>Marcin</h4> | <h4>Marcin</h4> | ||
| - | Task 1: Prepare bacterial culture to isolate <a href="http://partsregistry.org/Part:BBa_K177044" style="color: black">BBa_K177044</a> | + | Task 1: Prepare bacterial culture to isolate <a href="http://partsregistry.org/Part:BBa_K177044" style="color: black">BBa_K177044</a> from <a href="http://partsregistry.org/Part:pSB1A3 style="color:black">pSB1A3</a>. |
Task 2: Another ligation of [http://partsregistry.org/Part:BBa_K177044<span style="color: black">BBa_K177044</span>] | Task 2: Another ligation of [http://partsregistry.org/Part:BBa_K177044<span style="color: black">BBa_K177044</span>] | ||
Revision as of 21:21, 9 July 2010
Main topic
Marcin
Task 1: Prepare bacterial culture to isolate BBa_K177044 from pSB1A3. Task 2: Another ligation of [http://partsregistry.org/Part:BBa_K177044BBa_K177044] Methods: * Ligation mixtures composition:10 μl digested insert 8 μl digested vector 5 μl Tango buffer(Fermentas) 2 μl ATP 10 mM solution 4 μl MQ water 1 μl ligase T4 (Fermentas)* Ligation was carried out about 20 hours and subsequently inactivated
