Team:Tokyo Metropolitan/Project/Fiber/Protocol
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Revision as of 09:07, 26 October 2010
E.coli Fiber Project Protocol
Protocol1:Grow up a culture of A.xylinum
Material
Equipment
Procedure
Note
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Protocol2:Grow up a culture of E.coli
Material
Equipment
Procedure
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Protocol3:PCR
Material
Equipment
Procedure
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Protocol4:Agarose gel electrophoresis
Material
Equipment
Procedure
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Protocol5-1:DNA purification from agarose gel
Material
Equipment
Procedure
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Protocol5-2:DNA Purification with silica gel
Material
- Binding buffer
- silica gel
- wash buffer
- TE buffer
Equipment
- centrifuge
- vortex
- aspirator
- pipette
- pipette tip
Procedure
- Add 3 times Binding buffer than digestion production
- Add 10µl of silica gel and mix with Vortex
- Centrifuge 1min
- Remove supernatant with aspirator
- Add Wash buffer and mix with vortex
- Centrifuge 30sec
- Remove supernatant with aspirator
- Remove ethanol by drying
- Add TE buffer and mix with Vortex
- Centrifuge 30sec
- DNA is solved in Supernatant
Protocol6:Restriction enzyme digestion
Material
Equipment
Procedure
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Protocol7:Ligation
Material
Equipment
Procedure
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Protocol8:Transformation
Material
Equipment
Procedure
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Protocol9:Miniprep (extraction of plasmid kit)
Material
Equipment
Procedure
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