Team:TU Delft/Project/sensing/characterization


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Characterization of the Sensing Parts


Characterized Strains


  • PalkS / AlkS: E.coli K12/310C
  • PalkS / AlkS / PalkB: E.coli K12/311C
  • P(CaiF) / AlkS / PalkB: E.coli K12/312C
  • Negative control: E.coli K12

The characterization process of the hydrocarbon sensing sub-project will entail output measurements of fluorescence at varying octane concentrations (0.1% – 1%) of the above mentioned strains. These measurements will be performed using a 96-well plate reader. Growth curves will be followed by OD-600 alongside the fluorescence measurements. The obtained fluorescence and growth measurements will be analyzed and modeled accordingly.

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