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Team:Newcastle/Meetings/26 July 2010
From 2010.igem.org
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# Gibson protocol | # Gibson protocol | ||
#* ''rocF'' to require 6 pair of primers, therefore try doing up 2 fragments first | #* ''rocF'' to require 6 pair of primers, therefore try doing up 2 fragments first | ||
| + | # Protocol for ''yneA'' | ||
| + | #* Do up the characterization separately, one bacteria to contain "yneA" to show filamentous, the other to have the ''lacI''. | ||
| + | #* Could have nice photo with the different morphology on agar | ||
| + | #* Do not shine UV light on during the gel extraction step to avoid DNA mutation | ||
| + | #* Make up lots of DNA to allow better transformation | ||
==Lab feedback== | ==Lab feedback== | ||
Revision as of 14:48, 27 July 2010
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Contents |
Roll call
Everyone here except Colin Harwood, Colin Davie and Wendy Smith
Research feedback
- Levan glue - Levan requires sucrose to work
- Test how much sucrose is needed - Phil
- Kill switch - Use of mazE and mazF
- How much sucrose is needed for it to work?
- How fast does the bug use up all the sucrose?
- Stochastic switch - Use of hinC as the invertase
- Matt to work with Phil to work out the probability of the switching
- Have to work out what is wrong with the iGEM 2009 stochastic switch first
- Gibson protocol
- rocF to require 6 pair of primers, therefore try doing up 2 fragments first
- Protocol for yneA
- Do up the characterization separately, one bacteria to contain "yneA" to show filamentous, the other to have the lacI.
- Could have nice photo with the different morphology on agar
- Do not shine UV light on during the gel extraction step to avoid DNA mutation
- Make up lots of DNA to allow better transformation
Lab feedback
- Arginine test - Alan and Harsh
- Test worked fine.
- Could refine the test by using universal liquid pH indicator or to use the pH meter.
- DNA extraction - Harsh and Racheal
- Could only confirm the result on 27.07.2010.
Wiki
- Not able to do the drop down menue with the shadow test, therefore could try using htm5.
- Horizontal strip to change to purple.
Visas
- Deena to travel to London on 12.08.2010.
Action point
- Talk to Lula - Phil and Harsh
- Email to iGEM HQ to get stickers and badges - Harsh
- Hyperlink the protocol page to the lab notebook
- Lab wiki checker - Alan and Harsh
- Send reminder to Jen to add Jane to the mailing list - Jannetta
- Put CBCB and Biomedical school logo onto the wiki - Janetta
- Write to Chris Voigt to get the source code - Steven
- Tutorial for Inkscape - Jannetta
- To check if the extra USA visa immigration form is valid for 2 years - Jen
- Remind Neil about the MrGene logo - Steven
Next meeting
- Phil is chair, Alan is computer, Racheal is minutes
- Wednesday afternoon, 3pm.
   
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