From 2010.igem.org

Arginine is an amino acid that is acidic. Therefore if B. subtilis 168 is able to take up arginine, it will cause a pH change in the media. This would result in an increase in pH.

Control (1) - No change in pH
Control (2) - No change in pH
Control (3) - Increase in pH, however will be lower than test 1 and test 2.
Test (1) - Increase in pH
Test (2) - Increase in pH

For actual results, please see 23.07.10 lab results.

Conclusion

For experimental conclusion, please see 23.07.2010 lab results.

@@ Line 1: / Line 1: @@
-===Chromosomal DNA Extraction of ''B. Subtilis'' Strain 168 and 3610===
+{{Team:Newcastle/mainbanner}}
-Aim:
+=Arginine Test=
-Materials: LB Broth, ''B. Subtilis'' Strain 168 colonies and ''B. Subtilis'' Strain 3610 colonies,
+==Aim==
+The aim of this experiment is to determine whether ''B. subtilis'' 168 is able to take up external arginine.
-Procedures:
+==Procedure==
+* Please refer to [[Team:Newcastle/Arginine test| Arginine test]]
-# 5ml of LB Broth is pipetted into a universal tube, two tubes of each strain were prepared.
+==Expected results==
-# Loop is heated to pick colonies from agar plates.
+Arginine is an amino acid that is acidic. Therefore if ''B. subtilis'' 168 is able to take up arginine, it will cause a pH change in the media. This would result in an increase in pH.
-# All tubes are left overnight in the 37°C room.
+#Control (1) - No change in pH
+#Control (2) - No change in pH
+#Control (3) - Increase in pH, however will be lower than test 1 and test 2.
+#Test (1) - Increase in pH
+#Test (2) - Increase in pH
+For actual results, please see [[Team:Newcastle/23_July_2010|23.07.10]] lab results.
+==Conclusion==
+For experimental conclusion, please see 23.07.2010 lab results.
+{{Team:Newcastle/footer}}

Team:Newcastle/22 July 2010

From 2010.igem.org

Latest revision as of 21:47, 25 October 2010

Contents

Arginine Test

Aim

Procedure

Expected results

Conclusion