Arginine Test

Aim

The aim of this experiment is to determine whether B. subtilis 168 is able to take up external arginine.

Procedure

• Please refer to Arginine test

Expected results

Arginine is an amino acid that is acidic. Therefore if B. subtilis 168 is able to take up arginine, it will cause a pH change in the media. This would result in an increase in pH.

1. Control (1) - No change in pH
2. Control (2) - No change in pH
3. Control (3) - Increase in pH, however will be lower than test 1 and test 2.
4. Test (1) - Increase in pH
5. Test (2) - Increase in pH

For actual results, please see 23.07.10 lab results.

Conclusion

For experimental conclusion, please see 23.07.2010 lab results.