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Contents 1 rocF BioBrick miniprep 2 rocF BioBrick nanodrop 3 rocF BioBrick gel electrophoresis 4 Subtilin Immunity BioBrick 4.1 Aims 4.2 Materials and protocol 4.3 Results 4.4 Discussion 4.5 Conclusion

rocF BioBrick miniprep

rocF BioBrick nanodrop

rocF BioBrick gel electrophoresis

Expect band to be 3195bp.

Subtilin Immunity BioBrick

Aims

We plan to select three spaIFEG gene (part 3) PCR tubes, using Tms 51°C, 56°C and 61°C (we had confirmed it successful from yesterday's gel electrophoresis). Gel extraction will then be performed, along with Plasmid Vector (part 1), Promoter & RBS (part 2) and Double terminator (part 4). Finally, NanoDrop will be performed.

Materials and protocol

Please refer to the gel electrophoresis, gel extraction and NanoDrop protocols.

Results

Discussion

Conclusion

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