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Contents 1 Subtilin Immunity BioBrick 1.1 Aims 1.2 Materials and protocol 1.3 Results 1.4 Discussion

Subtilin Immunity BioBrick

Aims

Our aims for today are to run the gel electrophoresis to check whether we have the correct fragment sizes on the four parts that are we amplified yesterday. If the PCR worked, we will then perform gel extraction and then perform another gel electrophoresis for the extracted gel in order to obtain our BioBrick parts.

Materials and protocol

Please refer to the gel electrophoresis and the gel extraction protocols.

Results

Figure 1: Gel electrophoresis of the PCR products of the parts required for the subtilin immunity BioBrick.

• Lane 1: 1 kb DNA ladder
• Lane 2: Plasmid Vector (pSB1C3)
• Lane 3: Promoter and RBS (pVeg-SpoVG)
• Lane 4: spaIFEG Gene Cluster
• Lane 5: Double terminator
• Lane 6: 100 bp DNA ladder

Discussion

Plasmid Vector (lane 2), Promoter & RBS (lane 3) and Double terminator (lane 5) showed up. spaIFEG PCR tube (lane 4) did not show up. We think that this occurrence was due to the Tm error (it should be 63°C as opposed to 46°C). Therefore, another PCR and gel electrophoresis were performed. Please see tomorrow's lab book page for this.

Go back to our main Lab book page