Team:KIT-Kyoto/Parts

From 2010.igem.org

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{{Template:KIT-Kyoto/Parts1}}
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== We use these parts for making original biobrick parts ==
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=== 1.We get these parts from 2010 iGEM kit ===
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{| style="margin: 1em auto 1em auto" width="750px"
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|- style="background-color:#c1e4e9;"
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!Name||Part type||Resistance||Insert||Vector||Contents
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB3k3</partinfo>(<partinfo>BBa_J04450</partinfo>)||Plasmid backbone||Kanamycin||738bp||2750bp||promoter(LacI) +RBS+mRFP+T+T
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|-  style="background-color:#eaf4fc;"
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|<partinfo>pSB6A1</partinfo>(<partinfo>BBa_J04450</partinfo>)||Plasmid backbone||Ampicillin||738bp||4032bp||promoter(LacI) +RBS+mRFP+T+T
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1C3</partinfo>(<partinfo>BBa_J04450</partinfo>)||Plasmid backbone||Chloramphenicol||1069bp||2072bp||promoter(LacI) +RBS+mRFP+T+T
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I3522</partinfo>)||Composite||Ampicillin||937bp||2079bp||promoter(TetR)+RBS+GFP+T+T
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_R0040</partinfo>)||Regulatory||Ampicillin||54bp||2079bp||promoter(TetR)
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I732019</partinfo>)||Regulatory||Ampicillin||3230bp||2079bp||RBS+LacZ+T+T
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1AK3</partinfo>(<partinfo>BBa_I732950</partinfo>)||Protein domain||Kanamycin||3230bp||2079bp||RBS+LacZ+T+T
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB4A5</partinfo>(<partinfo>BBa_K193602</partinfo>)||Composite||Ampicillin||1896bp||3395bp||promoter(Lac)+RBS+melA+T+T|
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0240</partinfo>)||Protein domain||Ampicillin||883bp||2079bp||RBS+GFP+T+T
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13507</partinfo>)||Protein domain||Ampicillin||937bp||2079bp||RBS+RFP+T+T
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0420</partinfo>)||Protein domain||Ampicillin||878bp||2079bp||RBS+CFP+T+T
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0430</partinfo>)||Protein domain||Ampicillin||878bp||2079bp||RBS+YFP+T+T
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13600</partinfo>)||Composite||Ampicillin||940bp||2079bp||promoter(TetR)+RBS+CFP+T+T
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|- style="background-color:#eaf4fc;"
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|<partinfo>pSB1A2</partinfo>(<partinfo>BBa_J22005</partinfo>)||Composite||Ampicillin||2079bp||2623bp||promoter(TetR)+RBS+YFP+T+T
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|-
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|}
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=== 2.We get this part directly from iGEM HQ ===
 +
We characterize this BioBrick Part and enter the new information back on the Registry. This parts don’t include detailed information on pSB6 and the reasons leading to its usage in our work to produce GFP. In general, pSB6 is a low copy plasmid which makes it more suitable for protein production than the high copy vector. However this information cannot be found in any sites related to iGEM. Consequently, we tried to compare the performances of the low copy vector pSB1 and pSB6. We have confirmed in this way that pSB6 is more efficient at producing GFP protein than PSB1. We have thus proved that this part is very useful to evaluate the capabilities of a promoter, in this case by observing the degree of fluorescence of GFP protein. We are very thankful to the 08’Tokyo-Tech group who has supplied us with this part.
 +
 
 +
{| style="margin: 1em auto 1em auto" width="750px"
 +
|- style="background-color:#c1e4e9;"
 +
!Name||Part Type||Resistance||Insert||Vector||Contents|
 +
|- style="background-color:#eaf4fc;"
 +
|<partinfo>pSB6A1</partinfo>(<partinfo>BBa_K121013</partinfo>)||Protein domain||Ampicillin||883bp||4022bp||RBS+GFP+T+T
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|}
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== We make these parts originally. ==
== We make these parts originally. ==

Revision as of 03:56, 23 October 2010



Home > Parts
Language : English / Japanese

We use these parts for making original biobrick parts

1.We get these parts from 2010 iGEM kit

NamePart typeResistanceInsertVectorContents
<partinfo>pSB3k3</partinfo>(<partinfo>BBa_J04450</partinfo>)Plasmid backboneKanamycin738bp2750bppromoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB6A1</partinfo>(<partinfo>BBa_J04450</partinfo>)Plasmid backboneAmpicillin738bp4032bppromoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB1C3</partinfo>(<partinfo>BBa_J04450</partinfo>)Plasmid backboneChloramphenicol1069bp2072bppromoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I3522</partinfo>)CompositeAmpicillin937bp2079bppromoter(TetR)+RBS+GFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_R0040</partinfo>)RegulatoryAmpicillin54bp2079bppromoter(TetR)
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I732019</partinfo>)RegulatoryAmpicillin3230bp2079bpRBS+LacZ+T+T
<partinfo>pSB1AK3</partinfo>(<partinfo>BBa_I732950</partinfo>)Protein domainKanamycin3230bp2079bpRBS+LacZ+T+T
<partinfo>pSB4A5</partinfo>(<partinfo>BBa_K193602</partinfo>)CompositeAmpicillin1896bp3395bp
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0240</partinfo>)Protein domainAmpicillin883bp2079bpRBS+GFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13507</partinfo>)Protein domainAmpicillin937bp2079bpRBS+RFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0420</partinfo>)Protein domainAmpicillin878bp2079bpRBS+CFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0430</partinfo>)Protein domainAmpicillin878bp2079bpRBS+YFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13600</partinfo>)CompositeAmpicillin940bp2079bppromoter(TetR)+RBS+CFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_J22005</partinfo>)CompositeAmpicillin2079bp2623bppromoter(TetR)+RBS+YFP+T+T

2.We get this part directly from iGEM HQ

We characterize this BioBrick Part and enter the new information back on the Registry. This parts don’t include detailed information on pSB6 and the reasons leading to its usage in our work to produce GFP. In general, pSB6 is a low copy plasmid which makes it more suitable for protein production than the high copy vector. However this information cannot be found in any sites related to iGEM. Consequently, we tried to compare the performances of the low copy vector pSB1 and pSB6. We have confirmed in this way that pSB6 is more efficient at producing GFP protein than PSB1. We have thus proved that this part is very useful to evaluate the capabilities of a promoter, in this case by observing the degree of fluorescence of GFP protein. We are very thankful to the 08’Tokyo-Tech group who has supplied us with this part.

NamePart TypeResistanceInsertVector
<partinfo>pSB6A1</partinfo>(<partinfo>BBa_K121013</partinfo>)Protein domainAmpicillin883bp4022bpRBS+GFP+T+T


We make these parts originally.

<groupparts>iGEM010 KIT-Kyoto</groupparts>


Retrieved from "http://2010.igem.org/Team:KIT-Kyoto/Parts"