Team:HokkaidoU Japan/Notebook/September9

From 2010.igem.org

(Difference between revisions)
(→HSP, GFP, pSB1C3の3 piece ligation)
(→3 piece ligation of HSP, GFP and pSB1C3)
Line 7: Line 7:
|-
|-
| HSP
| HSP
-
| PCRしたもの
+
| PCRed
-
| ゲル抽
+
| Gel extracted
| Digestion
| Digestion
|-
|-
| GFP
| GFP
-
| 前日にゲル抽キットで精製した
+
| Previously purified by gel extraction
| －
| －
| Digestion
| Digestion
|-
|-
| pSB1C3
| pSB1C3
-
| PCR後，ゲル抽精製したもの
+
| Previously purified by gel extraction
| －
| －
| Digestion
| Digestion
Line 23: Line 23:
|}
|}
-
* HSPのゲル抽のついでにGFPを電気泳動したところ，薄かったためこれもゲル抽することにした
+
* Electrophoresed HSP and the same time also did GFP electrophoresis
-
* HSPとGFPのゲル抽後，電気泳動したところ，どちらも今後の操作には薄すぎたため，後日PCRからやりなおすことにした
+
* GFP band was very weak
 +
* After gel extraction electrophoresed both both HSP and GFP
 +
* Now both bands were too weak
 +
* Retry PCR next day

Revision as of 05:56, 28 September 2010

Notebook

3 piece ligation of HSP, GFP and pSB1C3

HokkaidoU Japan 20100909a.jpg
HSP PCRed Gel extracted Digestion
GFP Previously purified by gel extraction － Digestion
pSB1C3 Previously purified by gel extraction － Digestion
  • Electrophoresed HSP and the same time also did GFP electrophoresis
  • GFP band was very weak
  • After gel extraction electrophoresed both both HSP and GFP
  • Now both bands were too weak
  • Retry PCR next day
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Notebook/September9"