Team:HokkaidoU Japan/Notebook/September9

From 2010.igem.org

3 piece ligation of HSP, GFP and pSB1C3

PCR of products after gel extraction
HSP PCRed Gel extracted Digestion
GFP Previously purified by gel extraction Digestion
pSB1C3 Previously purified by gel extraction Digestion
  • Electrophoresed HSP and the same time also did GFP electrophoresis
  • GFP band was very weak
  • After gel extraction electrophoresed both both HSP and GFP
  • Now both bands were too weak
  • Retry PCR next day