Team:HokkaidoU Japan/Notebook/September9
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Revision as of 05:57, 28 September 2010
3 piece ligation of HSP, GFP and pSB1C3
HSP | PCRed | Gel extracted | Digestion |
GFP | Previously purified by gel extraction | - | Digestion |
pSB1C3 | Previously purified by gel extraction | - | Digestion |
- Electrophoresed HSP and the same time also did GFP electrophoresis
- GFP band was very weak
- After gel extraction electrophoresed both both HSP and GFP
- Now both bands were too weak
- Retry PCR next day